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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IH IF

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase alpha-1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.
Catalog #	A4000-52A
Applications	Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60°C is sufficient).
	Immunohistochemistry (Paraffin): 1:200
	Immunofluorescence: 1:100-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3C114
Host	Mouse
Source	Rabbit
Concentration	Not Determined
Form	Supplied as a liquid, 0.1% sodium azide.
Purity	Ascities
Immunogen	Purified Na,K-ATPase from rabbit renal outer medulla
Specificity	Recognizes rabbit Na,K-ATPase alpha 1 subunit. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.