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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase alpha 1, Na, K

Anti -ATPase alpha 1, Na, K

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH IF
Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase alpha-1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.
Catalog #A4000-52A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60°C is sufficient).
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3C114
HostMouse
SourceRabbit
ConcentrationNot Determined
FormSupplied as a liquid, 0.1% sodium azide.
PurityAscities
ImmunogenPurified Na,K-ATPase from rabbit renal outer medulla
SpecificityRecognizes rabbit Na,K-ATPase alpha 1 subunit. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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