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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E IP IH

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
Catalog #	A4000-52E
Applications	Suitable for use in ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:1000. Staining in rat liver yeilds a pattern consistent with plasma membrane localization.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8J326
Host	Mouse
Source	Rat
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Purity	Ascites
Immunogen	Purified rat kidney sodium/potassium ATPase. Epitope: Within an intracellular region at or near Asp-369 of the sodium/potassium ATPase alpha 1 subunit.
Specificity	Recognizes rat ATPase alpha 1, Na, K. Species Crossreactivity: human, canine and chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.