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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase beta 1, Na, K

Anti -ATPase beta 1, Na, K

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (approximate residues 62-304) of the beta subunit.
Catalog #A4000-71
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Immunoblot Analysis: A 1:2500 dilution detects the b-1 subunit in 50ug of non-reduced brain (~48kD) and kidney (~52kD) microsomal preparations. To reduce background, wash the blot with several changes of buffer containing 0.1% Tween-20, then with a buffer containing 0.5M sodium chloride.
Immunoprecipitation: 5ul immunoprecipitates the b-1 subunit from rat brain microsomal preparation.
Optimal dilutions to be determined by the researcher.
*Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.05 sodium azide, before the addition of glycerol to 40%.
PuritySerum
ImmunogenFusion protein corresponding to aa152-304 of the rat beta 1 subunit, as deduced from the cDNA.
SpecificityRecognizes rat beta 1 subunit at 48/52kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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