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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IP IH

The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (~residues 62-304) of the beta subunit.
Catalog #	A4000-72B
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:250
	Immunohistochemistry: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6D136
Host	Mouse
Source	Sheep
Concentration	Not Determined
Form	Supplied as a liquid PBS, 0.05% sodium azide.
Purity	Supernatant
Immunogen	Purified sheep kidney Na+/K+ ATPase-beta.
Specificity	Recognizes the beta1 subunit of sheep Na+/K+ ATPase-beta. Species Crossreactivity: human, mouse, porcine and canine
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.