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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase beta 2, Na, K

Anti -ATPase beta 2, Na, K


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Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Catalog #A4000-75
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Detects beta 2 in 20ug of a brain microsomal preparation using chemiluminescence. To reduce background, wash the blot with several changes of buffer containing 0.1% Tween-20, then with a buffer containing 0.5M sodium chloride.
Immunohistochemistry: 1:200 (formaldehyde fixed tissue). Reported by an independent laboratory.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50?% lycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid.
PurityNeat serum
ImmunogenBeta 2 subunit fusion protein containing residues 63-285, as deduced from cDNA. The beta 2 subunit exhibits virtual identity with the glial cell adhesion molecule AMOG.
SpecificityRecognizes rat Na, K-ATPase beta 2 subunit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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