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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase, Na, K

Anti -ATPase, Na, K

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Highly Purified E B
Catalog #A4000-84
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, and conjugation. Other applications not tested.
Recommended DilutionsWestern Blot: Reactive against the intact enzyme and enzyme subunits (alpha at 100 kD and beta at 50 kD) as well as glycosylated and non-glycosylated enzyme forms. A dilution of 1:100 to 1:500 is suggested for immunoblot followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room
temperature.
ELISA: 1:1,000 to 1:6,000, 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) and (ABTS (2,2-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceCanine
Concentration~10mg/ml
FormSupplioed as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
ImmunogenATPase, Sodium, Potassium (Canine Kidney)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-chicken serum as well as purified and partially purified ATPase, sodium, potassium (Canine Kidney).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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