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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IC

Catalog #	A4000-88
Applications	Suitable for use in Immunocytochemistry. Immunoblot is not recommended. Other applications not tested.
Recommended Dilution	Immunocytochemistry: Reported to detect ATP synthase
	(mitochondrial), beta chain in cultured rat neurons and glial cells fixed in 0.05% glutaraldehyde in PBS for 20 minutes, followed by 4% paraformaldehyde for 15 minutes. Cells were then permeabilized in 0.5% Triton X-100 in 2% horse serum and 2% fetal bovine serum in PBS. Cells were labeled with 1ug/ml anti-ATP Synthase (mitochondrial), beta chain and visualized with Alexa 488 goat anti-mouse IgG.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3J2
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact rat mitochondria.
Specificity	Recognizes ATP Synthase (mitochondrial), beta chain. Species Crossreactivity: Human, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.