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You are here:Home » Antibodies » Abs to Enzymes, Synthase » Anti -ATP Synthase, beta, Mitochondrial

Anti -ATP Synthase, beta, Mitochondrial

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
Catalog #A4000-88
ApplicationsSuitable for use in Immunocytochemistry. Immunoblot is not recommended. Other applications not tested.
Recommended DilutionImmunocytochemistry: Reported to detect ATP synthase
(mitochondrial), beta chain in cultured rat neurons and glial cells fixed in 0.05% glutaraldehyde in PBS for 20 minutes, followed by 4% paraformaldehyde for 15 minutes. Cells were then permeabilized in 0.5% Triton X-100 in 2% horse serum and 2% fetal bovine serum in PBS. Cells were labeled with 1ug/ml anti-ATP Synthase (mitochondrial), beta chain and visualized with Alexa 488 goat anti-mouse IgG.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3J2
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenIntact rat mitochondria.
SpecificityRecognizes ATP Synthase (mitochondrial), beta chain. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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