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You are here:Home » Antibodies » Antibodies-Enzymes, ATPase » Anti -ATPase 1, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA1)

Anti -ATPase 1, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA1)


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IF
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA 1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA 2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA 2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA 2b isoform expressed in all cell types. The SERCA 3 gene is not as well characterized and is found in non-muscle cells.
Catalog #A4000-91A
ApplicationsSuitable for use in Western Blot and Immunofluorescence. This antibody has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500. Immunofluorescence staining of SERCA 1 ATPase in canine skeletal muscle results in strong labeling of the entire type II (fast) myofiber.
Western Blot: 1:2500; Detects an ~110kD protein representing SERCA 1 ATPase in mouse muscle extracts.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4i390
ConcentrationAs reported
FormSupplied as a liquid in PBS with 0.05% sodium azide.
ImmunogenPurified rabbit skeletal muscle sarcoplasmic reticulum.
SpecificityRecognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum. Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase. Species Crossreactivity: Human, Rabbit, Mouse and Canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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