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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
Catalog #A4000-93
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000; Detects an ~110kD protein representing SERCA2 ATPase in rat cardiac tissue.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4i233
HostMouse
SourceCanine
ConcentrationAs reported
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityAscites
ImmunogenPurified canine cardiac sarcoplasmic reticulum vesicles.
SpecificityDetects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase. Recognizes the 2a isoform. Does not recognize 2b isoform. Species Crossreactivity: Human, Rabbit, Rat, Mouse, Canine and Guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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