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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF N
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
Catalog #A4000-94
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Blocking/Neutralization, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2500 Detects an ~110kD protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not suitble for Western Blot detection of rat SERCA2.
Immunohistochemistry (Frozen): 1:500
Immunohistochemistry (paraffin): 1:10-1:2000
Immunofluorescence (IC): 1:10-1:2000 Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I
(slow) myofiber consistent with sarcoplasmic reticulum localization.
Blocking/neutralization: 50-200 molar excess
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i351
HostMouse
SourceCanine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPuriifed by affinity chromatography.
ImmunogenPurified canine cardiac sarcoplasmic reticulum
SpecificityRecognizes canine Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. Does not discriminate between the two isoforms. Species Crossreactivity: human, rabbit, rat, bovine, porcine, mouse, amphibian, canine, guinea pig, hamster, primate, sheep and virus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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