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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs) mediate Ca2+ uptake into intracellular stores. SERCA mediated Ca2+ uptake induces and maintains muscular relaxation. SERCA1 is expressed in fast-twitch muscles, whereas SERCA2 is expressed in cardiac and slow-twitch muscles. SERCA2 plays an important part in regulating cardiac contractile function.
Catalog #A4000-94A
Applications
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5ug/ml detected SERCA2 in 10ug of HEK293 cell lysates
Optimal dilutions to be determined by the researcher.
Positive ControlHEK293 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No10H15
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified canine cardiac sarcoplasmic reticulum.
SpecificityRecognizes human ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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