ATP-Citrate Lyase, NT GST tag, Recombinant, Human (ACLY, ATP Citrate (pro-S) Lyase, ATP
Citrate Synthase, Citrate Cleavage Enzyme, EC 2.3.3.8)
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| ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. | | | Catalog # | A4001-35R | | Source | Recombinant corresponding to full length human ATP citrate lyase with N-terminal GST tag expressed in baculovirus expression system (NM_001096). | | Specific Activity | ~400pmole/min/ug. A | | Assay Condition | 25ul of ACLY reaction is conducted in a buffer containing 40mM Tris, pH 8.0, 10mM magesium chloride, 2mM DTT, 2mM potassium citrate, 0.1mM CoA, 6-100ng human ACLY at 30ºC for 60 minute. Enzyme activity was assayed using ADP-Glo reagents. | | Applications | Suitable for use in enzyme kinetics, inhibitor screening and selectivity profiling. Other applications not tested. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 147kD | | Source | Baculovirus | | Purity | Purified 90% | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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