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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IC IF

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATPase (soluble) and the F0 ATPase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta and epsilon subunits. While the F0 consists of a, b and c subunits.
Catalog #	A4005
Applications	Suitable for use in Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunocytochemistry: 1ug/ml for one hour at RT using 10e6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H385
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified
Immunogen	Rat brain mitochondria.
Specificity	Recognizes the beta chain of rat ATP Synthase. Species Crossreactivity: human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.