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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IF

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATPase (soluble) and the F0 ATPase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta and epsilon subunits. While the F0 consists of a, b and c subunits.
Catalog #	A4005-01A
Applications	Suitable for use in Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunofluorescence: 5ug/ml
	Immunoprecipitation: Detects an ~50kD protein representing ATP Synthase from solubilized rat brain mitochondria.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8J332
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
Purity	Purified
Immunogen	Intact rat mitochondria. Cellular Localization: Mitochondrial.
Specificity	Recognizes the beta subunit of rat ATP Synthase. Useful as a mitochondrial marker. Species Crossreactivity: Human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.