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You are here:Home » Antibodies » Abs to Enzymes, Synthase » Anti -ATP Synthase, beta

Anti -ATP Synthase, beta

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IF
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATPase (soluble) and the F0 ATPase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta and epsilon subunits. While the F0 consists of a, b and c subunits.
Catalog #A4005-01A
ApplicationsSuitable for use in Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 5ug/ml
Immunoprecipitation: Detects an ~50kD protein representing ATP Synthase from solubilized rat brain mitochondria.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J332
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified
ImmunogenIntact rat mitochondria. Cellular Localization: Mitochondrial.
SpecificityRecognizes the beta subunit of rat ATP Synthase. Useful as a mitochondrial marker. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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