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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, Itchy Homolog E3 Ubiquitin Protein Ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated Polypeptide 1, dJ468O1.1)

Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, Itchy Homolog E3 Ubiquitin Protein
Ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated Polypeptide 1, dJ468O1.1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral andpallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
Catalog #A4156-17
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide of AIF4.
SpecificityRecognizes human AIF4 at ~100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kD according to NP_113671.3). Species Crossreactivity: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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