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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy
Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB.
Catalog #A4156-17C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Brain (Frontal Cortex) lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
SpecificityRecognizes human AIF4. Species sequence homology: canine, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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