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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

Anti -Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy
Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB.
Catalog #A4156-17D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.03-0.1ug/ml, observed in human brain, frontal cortex, lysates on ~100kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-EIKSHDLKPNGGN, from human ITCH, at the internal region of the protein (NP_113671.3).
SpecificityRecognizes human ITCH. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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