Aurora A Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag
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| Human Aurora kinase A (GenBank Accession No. NM_003600), (aa 2-403) with N-terminal HIS6 tag, MW 50kD, expressed in an Baculovirus infected Sf9 cell expression system.. | | | Catalog # | A4189-01C | | Applications | Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. | | Storage and Stability | For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Specific Activity | ~ 120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C | | Assay buffer | 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na orthovanadate, 0.5mM ATP, 4uM aurora B substrate, and 0.4ug/ml Aurora A kinase. | | Source | Baculovirus infected Sf9 cells | | Purity | 90% by SDS-PAGE. | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 50% glycerol, 3mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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