Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)
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| Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system. | | | Catalog # | A4189-11 | | Stability | 6 months at –80 °C | | Purity | >95% by SDS-PAGE. | | Specific Activity | 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 °C. | | Assay buffer | 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase. | | Application | useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. | | Source | Recombinant, human from Baculovirus infected Sf9 cells | | Form | 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 3mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2) |
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