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You are here:Home » Molecular Biology » MB-Protein Kinases » Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)

Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine
Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)

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Specifications

Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system.
Catalog #A4189-11
Stability 6 months at 80 °C
Purity >95% by SDS-PAGE.
Specific Activity 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 °C.
Assay buffer 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase.
Application useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
SourceRecombinant, human from Baculovirus infected Sf9 cells
Form25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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