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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Aven (Apoptosis Caspase Activation Inhibitor, Cell Death Regulator Aven, PDCD12)

Anti -Aven (Apoptosis Caspase Activation Inhibitor, Cell Death Regulator Aven, PDCD12)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc. Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine Aspartase). The initiator caspases can be activated by self-cleavage. The effector caspases are then activated in an amplification cascade. Mitochondria play a pivotal role in the regulation of apoptosis. Several key factors are released from the mitochondria that regulate apoptosis. The first such factor (Cytochrome-C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1. Binding of the mitochondria factor allows Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9. Active caspase-9 then turns on downstream effector caspases, initiating apoptosis. Several key proteins regulate apoptosis by suppressing (e.g., Bcl-2 and related family of proteins) or promoting (e.g., AIF etc) apoptosis pathway. Bcl-XL is a known anti-apoptotic protein that acts at a late post-mitochondrial step. A new intracellular membrane protein, Aven (from Aventine, a Roman stronghold) has now been shown to bind both Bcl-XL and Apaf-1. Aven (human 362 aa) is a conserved protein that has broad tissue distribution with prominent expression in heart, skeletal muscle, kidney, liver, pancreas, testis, and several established cell lines (HeLa, IB4, and Raji). Aven interferes with the ability of Apaf-1 to self-associate, and subsequent inhibition of Apaf-1 mediated activation of caspases.
Catalog #A4445-11E
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. The predicted size of Aven is ~39kD. However, it migrates as 55kD in HeLa cell (1).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
Immunogen18-aa peptide from human Aven
SpecificityHuman control peptide has no significant sequence homology with other proteins. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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