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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
Catalog #	A4499-75
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 0.05-0.2ug/ml
	Western Blot: 0.1-1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to GLNDIFEAQKIEWHE from Avi tag conjugated to KLH.
Specificity	Recognizes C-terminal, N-terminal and internal Avi tagged fusion proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.