Avidin CAS 1405-69-2

Brand

BioSeparation™

CAS Number

1405-69-2

Grade

Molecular Biology Grade

Swiss Prot

P02701

Gene ID

396260

Molecular Weight

6668

EU Commodity Code

38220090

Shipping Temp

Blue Ice

Storage Temp

4°C

Avidin (Biotin Binding Protein) is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding log constant of 15. Avidin is a tetrameric protein, composed of subunits of identical amino acid composition and sequence. Each monomer is glycosylated on Asn-17.

The high affinity constant of Avidin for Biotin has been widely used in molecular biology: affinity chromatography, molecular recognition and labelling, Enzyme Linked ImmunoSorbent Assay (ELISA), histochemistry and cytochemistry. An excellent review in the field has been written by Wilchek, M. and Bayer, E.A., eds. (1990), 'Avidin-Biotin Technology', Methods in Enzymology, Vol.184, Academic Press.

Molecular Weight

Monomer at 16.5kD. Dimer at 33kD and Tetramer at 66kD may also appear under incomplete denaturation.

Source

Egg white

Purity

Purified by chromatography.

Protein

≥0.90 mg protein/mg solid

Protein Purity (10mg)

Homogeneous by SDS

Form

Supplied as a lyophilized powder.

Activity

≥12u/mg

Unit Definition

One unit will bind 1ug of d-biotin at pH 8.9.

Water

≤ 5%

Storage and Stability

Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at 4°C. Reconstituted product is stable for 6 months at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Source

Egg white

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

Berger, M., and Wood, H.: Purification of the Subunits of Transcarboxylase by Affinity Chromatography on Avidin-Sepharose, J. Biol. Chem., 250, 927 (1975).|Fraenkel-Conrat, H., Snell, N., and Ducay, E.: Avidin. I. Isolation and Characterization of the Protein and Nucleic Acid, Arch. Biochem. Biophys., 39, 80 (1952).|Green, N.: Avidin. 3. The Nature of the Biotin-Binding Site, Biochem. J., 89, 599 (1963a).|Green, N.: Purification of Avidin, in Methods in Enzymology, XVIII, (McCormick, D., and Wright, L., eds.), Academic Press, NY, 414 (1970a).|Green, N.: The Molecular Weight of Avidin, Biochem. J., 92, 16c (1964).|Green, N.: A Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin, Biochem. J., 94, 23c (1965).|Melamed, M., and Green, N.: Avidin. 2. Purification and Composition, Biochem. J., 89, 591 (1963).|Wei, R., and Wright, L.: Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin, Proc. Soc. Exp. Biol. Med., 117, 341 (1964).

USBio References

No references available

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A4500 Avidin (Egg White, 68kD Glycoprotein) CAS: 1405-69-2

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