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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Avidin

Anti -Avidin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IC
Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits. Each subunit is glycosylated at 17-Asparagine.
Catalog #A4500-06
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:20,000
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPuri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
ImmunogenAvidin, Hen Egg White
SpecificityRecognizes chicken Avidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as puri ed and partially puri ed Avidin. Does not react with Streptavidin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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