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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IC

Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits. Each subunit is glycosylated at 17-Asparagine.
Catalog #	A4500-06
Applications	Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:20,000
	Western Blot: 1:1000-1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	~10mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Immunogen	Avidin, Hen Egg White
Specificity	Recognizes chicken Avidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as puri ed and partially puri ed Avidin. Does not react with Streptavidin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.