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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Avidin (Fluorescein)

Anti -Avidin (Fluorescein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified B IF
Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits.
Catalog #A4500-40
ApplicationsSuitable for use in FLISA, Immunofluorescence, and Western Blot. Other applications have not been tested.
Recommended DilutionFLISA: >1:20,000
Western Blot: >1:10,000
Immunofluorescence: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength 528nm
Fluorochrome/Protein Ratio3.0 moles Fluorescein/mole Rabbit IgG
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Fluorescein conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and protease free). 0.01% sodium azide. Labeled with Fluorescein.
PurityPuri ed by Ion exchange chromatography.
ImmunogenAvidin (Hen Egg White)
SpecificityRecognizes chicken Avidin. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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