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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IF

Avidin, synthesized in the hen oviduct, is a glycoprotein of MW 68,000 daltons which occupies about 0.05% of the total protein content of the hen egg white. Native avidin is a tetrameric protein composed of four identical subunits.
Catalog #	A4500-40
Applications	Suitable for use in ELISA, Immunofluorescence, and Western Blot. Other applications have not been tested.
Recommended Dilution	ELISA: >1:20,000
	Western Blot: >1:10,000
	Immunofluorescence: 1:500-1:2500
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	495nm
Emission Wavelength	528nm
Fluorochrome/Protein Ratio	3.0 moles DTAF/mole Rabbit IgG
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Fluorescein conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	~10mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and protease free). 0.01% sodium azide. Labeled with Fluorescein.
Purity	Puri ed by Ion exchange chromatography.
Immunogen	Avidin (Hen Egg White)
Specificity	Recognizes chicken Avidin. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.