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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IH

Catalog #	A4500-45
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:7000 to 1:27,000. Assayed against 1ug of Avidin [Hen Egg White] in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	~10mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate, 40% glycerol. Labeled with HRP.
Purity	Puri ed by delipidation, fractionation and ion exchange chromatography.
Immunogen	Avidin (Hen Egg White).
Specificity	Recognizes chicken Avidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as puri ed and partially puri ed Avidin (Hen Egg White). Crossreactivity against Avidin from other sources is unknown.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.