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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Avidin D (Biotin)

Anti -Avidin D (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified IH
Biotinylated Anti-Avidin D is an unusual molecule. It is capable of binding to Avidin D by two mechanisms, either through the antigen binding sites in the Fab portion of the antibody or through multiple biotin residues covalently attached to the protein. Although little is known about the mechanism of binding, this reagent appears to provide superior amplification of fluorescent signals compared to biotinylated "extender" molecules or anti-avidin alone. The steps involved in using this product are straightforward. After a biotinylated antibody has been introduced, cells or sections are labeled with Fluorescein Avidin DCS, followed by Biotinlyated Anti-Avidin D, and then a second labeling with Fluorescein Avidin DCS. This procedure results in the introduction of several more fluorochromes at the antigenic site in the tissue. Cell sorter analysis has shown that a several-fold increase in fluorescence can be achieved in some antibody labeling studies using Biotinylated Anti-Avidin D. Other applications of this unique reagent are currently under investigation.
Catalog #A4500-81F
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
ConcentrationNot determined
FormSupplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin.
PurityAffinity Purified
SpecificityRecognizes Avidin D.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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