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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Axin, an important regulator of beta-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. A novel gene, AXUD1 (AXIN 1 up-regulated), has enhanced expression in response to exogenously expressed AXIN1. It is expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocytes.
Catalog #	A4745
ApplicationsWestern Blotting	1-2ug/ml detectsAxin in RIPA lysates from Cos-1 cells transfected with full length, mouse axin. Lysates of Cos-1 cells untransfected (lane 1) and expressing transfected Axin (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Axin (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 898-912 (SQRKAGGGSAPPCDS) of mouse axin. The immunizing
sequence is identical in rat and has 13/15 identical amino acids in human.
Specificity	Recognizes recombinant mouse Myc-tagged Axin expressed in transfected cell lysate, Mr 130kD. Predicted to crossreact with rat and human based on sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.