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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

The Axin (axis inhibition) protein is an ~120-130kD negative regulator of Wnt pathway signaling. The Wnt pathway is essential for embryonic axis development in amphibians, Drosophila, and mammals; Axin has been shown to inhibit dorsal axial development. In vertebrates, Wnt signaling occurs when the frizzled receptor transduces a signal to the Dishevelled protein, triggering glycogen synthase kinase-3 (GSK-3) inactivation and the accumulation of b-catenin. Axin binds directly to GSK-3b, b-catenin, and the adenomatous polyposis coli (APC) tumor suppressor protein at separate binding sites; these four proteins form a tetrameric complex that regulates b-catenin stability and signaling.
Catalog #	A4747A
Applications	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 0.1-2.0ug/ml. Detects a band at ~120-130kD using Jurkat leukemia T cell lysates.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.
Specificity	Recognizes human Axin. On Western blots, it recognizes a doublet band at ~120-130kD. Species sequence homology: mouse, rat, and chicken-86%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.