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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Axin (Axis Ihibition Protein)

Anti -Axin (Axis Ihibition Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The Axin (axis inhibition) protein is an ~120-130kD negative regulator of Wnt pathway signaling. The Wnt pathway is essential for embryonic axis development in amphibians, Drosophila, and mammals; Axin has been shown to inhibit dorsal axial development. In vertebrates, Wnt signaling occurs when the frizzled receptor transduces a signal to the Dishevelled protein, triggering glycogen synthase kinase-3 (GSK-3) inactivation and the accumulation of b-catenin. Axin binds directly to GSK-3b, b-catenin, and the adenomatous polyposis coli (APC) tumor suppressor protein at separate binding sites; these four proteins form a tetrameric complex that regulates b-catenin stability and signaling.
Catalog #A4747A
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.1-2.0ug/ml. Detects a band at ~120-130kD using Jurkat leukemia T cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.
SpecificityRecognizes human Axin. On Western blots, it recognizes a doublet band at ~120-130kD. Species sequence homology: mouse, rat, and chicken-86%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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