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You are here:Home » Molecular Biology » MB-Proteins » Axl, Recombinant, Human, GST-Tag (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)

Axl, Recombinant, Human, GST-Tag (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL
Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene
AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)

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Specifications

Human AXL (GenBank Accession No. NM_021913), (a.a. 473-894) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.
Catalog #A4775-10
Specific Activity140 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Assay Buffer50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate, and 1ug/ml recombinant AXL.
ApplicationsUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight77kD
SourceBaculovirus infected Sf9 cells
Purity70% by SDS-PAGE
ConcentrationAs reported
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol and 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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