Axl, Recombinant, Human (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
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| Human AXL (GenBank Accession No. NM_021913), (a.a. 473-894) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. | | | Catalog # | A4775-10 | | Specific Activity | 140 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. | | Assay Buffer | 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate, and 1ug/ml recombinant AXL. | | Applications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Molecular Weight | 77kD | | Source | Baculovirus infected Sf9 cells | | Purity | 70% by SDS-PAGE | | Concentration | As reported | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol and 3mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Axl, Recombinant, Human (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07) |
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