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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Axl, phosphorylated (Tyr779) (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)

Anti -Axl, phosphorylated (Tyr779) (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL
Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene
AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Axl (also known as ARK, UFO and TYR07) is a receptor tyrosine kinase that contains extracellular regions composed of two IgG-related domains linked to two fibronectin type III repeats. Gas6, a ligand for Axl, induces Axl phosphorylation on multiple tyrosine residues. Phosphorylation of Axl at Tyr779 is associated with the activation of PI 3-kinase.
Catalog #A4776-10
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml detects Axl in Wl38 human lung cell line conditioned media-treated A172 human glioblastoma cell line.
Immunocytochemistry: 5-15ug/ml detects Axl in immersion fixed A172 human glioblastoma cell line with and without pervanadate co-staining with total Axl.
Immunohistochemistry: 5-15ug/ml detects Axl in immersion fixed paraffin embedded sections of human stomach.
Optimal dilution to be determined by researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human Axl Tyr779 site.
SpecificityRecognizes endogenous human Axl, phosphorylated at Tyr779. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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