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Azocoll Substrate (Substrate for Matrilysin, MMP7)
Azocoll Substrate (Substrate for Matrilysin, MMP7)
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| Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. The genes encoding human a and b -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are initially synthesized as inactive prodefensins with a signal peptide that is cleaved off by Matrilysin/MMP7 (a tissue metalloproteinase) to generate mature and bioactive defensin peptide. Matrilysin is expressed in Paneth cell granules together with perhaps more than 20 different -defensins (cryptidins). Disruption of the matrilysin gene prevents the normal posttranslational proteolytic activation of intestinal -prodefensins. | | | Catalog # | A4777 | | Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas. | | Azocoll substrate is General proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520nm) | >1.0; blank (520nm, 5mg/ml buffer, 10min at 37°C, pH 7.0): <0.3. Mesh: >100 (minimum 80%). | | Specific Activity | ~3000units/mg per min at 37°C, pH 7.5. EC 3.4.24.23. | | Unit Definition | One unit is defined as the amount of enzyme that will digest 1ug AZOCOLL Substrate | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 100-500ng/lane | | ELISA: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | For long-term storage, aliquot and store at -20°C. Light sensitive. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Purity | Purified | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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