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You are here:Home » Antibodies » Abs to Receptors » Anti -ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)

Anti -ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC IF
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed.
Catalog #A8510
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Western Blot: 1:50 Detects a band at ~42kD. A non-reduced sample treatment and SDS-PAGE followed by blotting on nitrocellulose or PDFM is recommended. Block with 10% non-fat dry milk.
Immunohistochemistry (frozen): 1:50 Acetone-fixed
ELISA: 20ug/ml using 100ul/well
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlRat hepatocytes
Recommended Negative ControlRat Kidney, pancreas, small intestine, colon
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G132
HostMouse
SourceRat
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCrude rat liver membrane extracts
SpecificityRecognizes a subunit-specific epitope on RHL-1 of rat ASGPR. Species crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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