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You are here:Home » Antibodies » Abs to CD Markers » Anti -B220 (B cell specific isoform of CD45)

Anti -B220 (B cell specific isoform of CD45)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Highly Purified IC
B220 (CD45R) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
B220 (CD45R) is required for t-cell activation through the antigen receptor. The first ptpase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.
Catalog #B0000-01
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 10ul/10e6 cells
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No5C12
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 500ul sterile 40-50% glycerol, dH2O.
PurityPurified by ion exchange chromatography.
ImmunogenPurified mouse LN B cells.
SpecificityRecognizes mouse B220.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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