Anti -BACE1 (Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving
Enzyme 1, BACE 1, APP beta Secretase, Aspartyl Protease 2, Asp 2, ASP2, Asp-2, Beta
Secretase 1, Beta Secretase, Beta Site Amyloid beta A4 Precursor Protein Cleaving Enzy
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
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B IP |
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| -secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP- (sAPP ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by -secretase generates the amyloid -peptide (A ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4). | | | Catalog # | B0002-88G | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:50. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10E7 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied in 10 mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol. | | Immunogen | Synthetic peptide corresponding to residues surrounding Asp490 of human BACE protein (KLH- coupled). | | Specificity | Recognizes human BACE. Species crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -BACE1 (Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1, BACE 1, APP beta Secretase, Aspartyl Protease 2, Asp 2, ASP2, Asp-2, Beta Secretase 1, Beta Secretase, Beta Site Amyloid beta A4 Precursor Protein Cleaving Enzy |
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