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You are here:Home » Antibodies » Abs to Apoptosis » Anti -BACE1 (26-45) (Beta-site APP Cleaving Enzyme)

Anti -BACE1 (26-45) (Beta-site APP Cleaving Enzyme)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
b-secretase, also called BACE (b-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease and in the formation of myelin sheaths in peripheral nerve cells. BACE1, also known as memapsin-2, is the major b-secretase for generation of the 40 or 42 amino-acid-long b-amyloid peptides that aggregate in the brain of Alzheimer's patients. Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by g-secretase. The second cleavage releases the intracellular domain of APP and b-amyloid.
The immature form of BACE1 contains an N-terminal signal sequence (residues 121) followed by a large catalytic domain, a single transmembrane domain (residues 461477), and a short cytoplasmic domain (residues 478501). The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein, which starts at residue 22. The proBACE1 protein is modified by cleavage of 12 N-terminal residues (aa 22-45), producing the mature BACE1 protein.
Catalog #B0002-91A3
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide [IRLPLRSGLGGAPLGLRLPR], corresponding to amino acids 26-45 at N-terminal of human BACE1. (KLH-conjugated)
SpecificityRecognizes human BACE1. Species sequence homology: mouse, rat and bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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