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Anti -BACE1 (46-65) (Beta-site APP Cleaving Enzyme)
Anti -BACE1 (46-65) (Beta-site APP Cleaving Enzyme)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| b-secretase, also called BACE (b-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease and in the formation of myelin sheaths in peripheral nerve cells. BACE1, also known as memapsin-2, is the major b-secretase for generation of the 40 or 42 amino-acid-long b-amyloid peptides that aggregate in the brains of Alzheimer's patients. Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by -secretase. The second cleavage releases the intracellular domain of APP and b-amyloid. The immature form of BACE1 contains an N-terminal signal sequence (residues 1–21) followed by a large catalytic domain, a single transmembrane domain (residues 461–477), and a short cytoplasmic domain (residues 478–501). The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein which starts at residue 22. The proBACE1 protein is modified by cleavage of 12 N-terminal residues (aa 22-45), producing the mature BACE1 protein. | | | Catalog # | B0002-91B1 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.05-0.2ug/ml | | Western Blot: 0.1-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.er. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide [ETDEEPEEPGRRGSFVEMVD], corresponding to amino acids 46-65 of human BACE1. (KLH-conjugated) | | Specificity | Recognizes human BACE1. Species sequence homology: mouse, rat and bovine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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