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You are here:Home » Antibodies » Antibodies-Apoptosis » Anti -BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

Anti -BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
Cleavage of amyloid precursor protein (APP) into 40 or 42 amino acid amyloid beta (ab) peptides is a result of the action of two proteases beta- and gamma-secretase. The beta-site APP cleaving enzyme, or BACE1, is a transmembrane aspartic protease that is localized in the Golgi and endosomes. Low levels of BACE1 mRNA are found in most adult tissues with a higher level of expression in pancreas.
Catalog #B0002-91C
ApplicationsSuitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 8ug/ml detects BACE1 in paraffin-embedded human Alzheimer’s brain tissue sections.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Western Blot: 2ug/ml detects human BACE1 in cell or tissue extracts under reducing conditions only.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Optimal dilutions to be determined by the researcher.
Western Blot BuffersBlotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20
Blocking Solution: 5% nonfat dry milk in Blotting Buffer
Antibody Solution: 5% nonfat dry milk in Blotting Buffer
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8H105
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa22-460 of human BACE1 expressed in NSO cells (P56817).
SpecificityRecognizes human BACE1. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesASP2, BACE, EC=, FLJ90568, HSPC104, KIAA1149, Memapsin-2

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