| Cleavage of amyloid precursor protein (APP) into 40 or 42 amino acid amyloid beta (ab) peptides is a result of the action of two proteases beta- and gamma-secretase. The beta-site APP cleaving enzyme, or BACE1, is a transmembrane aspartic protease that is localized in the Golgi and endosomes. Low levels of BACE1 mRNA are found in most adult tissues with a higher level of expression in pancreas. |
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| Catalog # | B0002-91C |
| Applications | Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: 8ug/ml detects BACE1 in paraffin-embedded human Alzheimer’s brain tissue sections. |
| Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. |
| Western Blot: 2ug/ml detects human BACE1 in cell or tissue extracts under reducing conditions only. |
| Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. |
| Optimal dilutions to be determined by the researcher. |
| Western Blot Buffers | Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20 |
| Blocking Solution: 5% nonfat dry milk in Blotting Buffer |
| Antibody Solution: 5% nonfat dry milk in Blotting Buffer |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8H105 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa22-460 of human BACE1 expressed in NSO cells (P56817). |
| Specificity | Recognizes human BACE1. Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | ASP2, BACE, EC=3.4.23.46, FLJ90568, HSPC104, KIAA1149, Memapsin-2 |
