Anti -BACE, Asp2 (Beta-secretase 1, Aspartyl Protease 2, ASP2, Asp 2, Beta-site Amyloid
Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1, Memapsin-2,
Membrane-associated Aspartic Protease 2, BACE1, KIAA1149)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B FC |
|
| beta-amyloid (Abeta) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, beta-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12kD CT membrane fragment. Alternatively, alpha-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble N-terminal fragment, APPsa, and a 10kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gamma-secretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as b-secretase. BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins. At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2, Asp3, and Asp4). Human BACE/Asp2/Memapsin2, located on chromosome 11, is a transmembrane protein of 501aa (signal peptide 1-21aa, a proprotein domain 22-45aa, 1 TM domain near the CT, and a short cytoplasmic CT- tail of 24aa; mature protein 46-460aa). The lumenal portion of BACE has two active site motifs at 93aa and 289aa with signature sequence of aspartic proteases. Rat and mouse BACE (501aa) are 96% identical with human BACE. BACE expression was most prominent in most areas of the rat brain and pancreas. It has been localized in the compartments of the secretory pathways. | | | Catalog # | B0002-91S | | Applications | Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: 1:10,000-100,000 | | Western Blot: 1-5ug/ml using chemiluminescence. BACE is ~70kD, greater than the theoretical size of ~51kD, due to glycosylation. | | Flow Cytometry: ~1ug/10e6 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11C83 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to human BACE, EC Domain. | | Specificity | Recognizes human BACE, asp2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -BACE, Asp2 (Beta-secretase 1, Aspartyl Protease 2, ASP2, Asp 2, Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1, Memapsin-2, Membrane-associated Aspartic Protease 2, BACE1, KIAA1149) |
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