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You are here:Home » Antibodies » Abs to Apoptosis » Anti -BACE2, Asp1 (Beta-Site APP Cleaving Enzyme 2)

Anti -BACE2, Asp1 (Beta-Site APP Cleaving Enzyme 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Catalog #B0002-92C
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. BACE is approx 70kD, greater than the theoretical size of ~51kD, due to glycosylation.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Immunohistochemistry & Immunofluorescence: Not tested. We recommend the use of 2:20ug/ml of affinity pure antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenA 20 aa peptide near the CT of human BACE2/Asp1 (1) conjugated to KLH
SpecificityThe human peptide sequence has high degree of homology (94%) with mouse and rat BACE/Asp2. No significant sequence homology exists with BACE2/Aps1, a homolog of BACE, or other Asps. Antibody crossreactivity in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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