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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Catalog #	B0003-05A
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components	the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Antibody Pair Recommendations	Use as the detection antibody in sandwich immunoassays with cat #B0003-05B as the capture antibody.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.B.10A
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 25% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified lethal factor from inactivated Bacillus anthracis culture. Vaccine strain STI-1.
Specificity	Recognizes lethal factor Bacillus anthracis. Does not crossreact with lethal factor antigen of Y. pestis, F. tularensis or Toxoplasma gondii.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.