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You are here:Home » Antibodies » Abs to Infectious Disease Anthrax » Anti -Bacillus anthracis (Anthrax)

Anti -Bacillus anthracis (Anthrax)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Catalog #B0003-05A
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Antibody Pair RecommendationsUse as the detection antibody in sandwich immunoassays with cat #B0003-05B as the capture antibody.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.10A
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 25% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified lethal factor from inactivated Bacillus anthracis culture. Vaccine strain STI-1.
SpecificityRecognizes lethal factor Bacillus anthracis. Does not crossreact with lethal factor antigen of Y. pestis, F. tularensis or Toxoplasma gondii.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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