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You are here:Home » Antibodies » Abs to Infectious Disease Anthrax » Anti -Bacillus anthracis LF (Lethal Factor) (Anthrax)

Anti -Bacillus anthracis LF (Lethal Factor) (Anthrax)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Catalog #B0003-05B
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Matched PairsCapture: B0003-05B
Detection: B0003-05A
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Antibody Pair RecommendationsUse B0003-05B as the capture antibody pair for sandwich immunoassays with B0003-05A as the detection antibody.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.10B
HostMouse
Concentration~1.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, with 0.1% sodium azide, before the addiition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified lethal factor from inactivated Bacillus anthracis culture (vaccine strain STI-1).
SpecificityRecognizes lethal factor antigen of Bacillus anthracis. Does not crossreact with protective antigen of B. anthracis, Y. pestis, F. tularensis or Toxoplasma gondii.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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