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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	B0003-05D
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components	the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Recommended Antibody Pair	B0003-05E (detection): Bacillus anthracis (Anthrax).
Hybridoma	Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	1.B.10D
Host	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide before the addition of glycerol to 40%.
Purity	Purified by Protein G Sepharose chromatography (> 95%).
Immunogen	Purified protective antigen from inactivated Bacillus anthracis culture (vaccine strain STI-1).
Specificity	Recognizes Protective Antigen of Bacillus anthracis. Does not crossreact with Lethal Factor Antigen of B. anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondii.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.