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You are here:Home » Antibodies » Abs to Infectious Disease Anthrax » Anti -Bacillus anthracis (Anthrax)

Anti -Bacillus anthracis (Anthrax)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #B0003-05E
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Recommended Antibody PairB0003-05D, B0003-05 Capture: Bacillus anthracis (Anthrax).
Detection: B0003-05E
Hybridoma:
Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No1.B.10E
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography (> 95%, SDS-PAGE).
ImmunogenPurified protective antigen from inactivated Bacillus anthracis culture (vaccine strain STI-1).
SpecificityRecognizes Protective Antigen of Bacillus anthracis. Does not crossreact with Lethal Factor Antigen of B. anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondii.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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