| After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker-8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kDa fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells. |
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| Catalog # | B0003-05G10 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. Recognizes ~90kD protein in Bacillus anthracis spore extract of Bacillus anthracis. |
| ELISA: 0.5-1ug/ml. Control antigen can be used to coat ELISA plates at 1ug/ml |
| Optimal dilution determined by the researcher. |
| Recommended Matched Pair | Capture: B0003-05G10 Bacillus anthracis Spore Antigen |
| Detection: B0003-05G11 Bacillus anthracis Spore Antigen |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 7C11 |
| Host | Mouse |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% sodium azide. Reconstitute in 100ul sterile dH2O. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Bacillus anthracis Spore Extract |
| Specificity | Recognizes Bacillus anthracis Spore extract antigen. Does not crossreact with proteins in Bacillus anthracis vegetative cells and B globuli, B. subtilis and B. cereus spores. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
