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You are here:Home » Antibodies » Abs to Infectious Disease Anthrax » Anti -Bacillus anthracis Spore Antigen (Anthrax) (Biotin)

Anti -Bacillus anthracis Spore Antigen (Anthrax) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #B0003-05G31
Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: Capture antibody in a sandwich ELISA
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended PairSuitable for use as the capture or solid phase antibody.
Matched to B0003-05G2 as the detection or conjugate (label) antibody.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G302A
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenB. anthracis spore extract
SpecificityRecognizes Bacillus anthracis spore antigen (92-94kD protein bands). No crossreactivity with B. anthracis vegetative cells, B. globigii, B. subtilis and B. cereus spores.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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