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You are here:Home » Antibodies » Abs to Infectious Disease Anthrax » Anti -Bacillus anthracis PA (Protective Antigen) (Anthrax)

Anti -Bacillus anthracis PA (Protective Antigen) (Anthrax)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. PA is secreted from Bacillus anthracis as an 83kD (PA83) protein which is cleaved by furin protease, releasing a 20kD fragment. The 63kD portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects.
PA is cleaved from the 83kD form to the 63kD form at aa164-167. Thus the antibody (made against aa186-192) should recognize both the 83kD and 63kD PA forms, but not the 20kD cleavage fragment.
Catalog #B0003-05M5
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by researcher.
Recommended Positive ControlPA recombinant protein
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptides corresponding to aa186-192 of the Anthrax PA protein (~83kD).
SpecificityRecognizes Anthrax PA (Protective Antigen) protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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