| Bacillus cereus is an endemic, soil-dwelling, Gram-positive, rod-shaped, beta hemolytic bacterium. Some strains are harmful to humans and cause foodborne illness, while other strains can be beneficial as probiotics for animals. B. cereus bacteria are facultative anaerobes, and like other members of the genus Bacillus can produce protective endospores. |
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| Bacillus subtilis, known as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions. Unlike several other well-known species, B. subtilis has historically been classified as an obligate aerobe, though recent research has demonstrated that this is not strictly correct. |
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| Catalog # | B0003-27A |
| Applications | Suitable for use in Immunofluorescence. Other applications not tested. |
| Recommended Dilutions | Immunofluorescence: 1:10-1:50. Acetone fixation of the antigen source is recommended prior to staining. |
| Optimal dilutions to be determined by the researcher. |
| Titer | >1:800 by IFA |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~4-5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). |
| Purity | Purified IgG fraction to a purity of 95%. |
| Immunogen | Purified spores of B. cereus (ATCC #11778) and B. subtilis (ATCC #9372). |
| Specificity | Recognizes Bacillus cereus/Bacillus subtilis spores. Antiserum is unabsorbed and is expected to crossreact with various Bacillus species. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
