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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -BAF53A (BAF Complex 53kD A Subunit, BRG1 Associated Factor, Actin-like 6A, ACTL6A, MGC5382)

Anti -BAF53A (BAF Complex 53kD A Subunit, BRG1 Associated Factor, Actin-like 6A, ACTL6A,
MGC5382)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
This protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53kD subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
Catalog #B0003-63M
Cellular Localization Nuclear
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:64,000
Western Blot: 1-3ug/ml. Predicted molecular weight 49kD. Detects a band of ~ 48kD ) in HeLa lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormTris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide
SpecificityExpected to recognize both BAF53A and BAF53B, which are almost identical. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) and Zebrafish (92% identity with immunogen) due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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