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You are here:Home » Antibodies » Abs to Chromatin Proteins » Anti -BAF57 (BRG1 Associated Factor 57, Mammalin Chromatin Remodeling Complex, SMARCE1, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin e1)

Anti -BAF57 (BRG1 Associated Factor 57, Mammalin Chromatin Remodeling Complex, SMARCE1,
SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin e1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The complex contains an ATP-dependent nucleosome disruption activity that can lead to enhanced binding of transcription factors. The BRG1/brm associated factors, or BAF, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF. A 57kD BAF subunit, BAF57, is present in higher eukaryotes, but not in yeast.
Catalog #B0003-63P
Cellular Localization Nuclear
Positive Control HeLa or Jurkat whole cell lysate.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000.
Western Blot: 1ug/ml. ~ 37kD band in Hela and Human Brain lysates.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: PPTDPIPEDEKKE, representing the C-terminus of the human protein (residues 399-411), according to NP_003070.
SpecificitySpecies Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (99% identity with immunogen) due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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