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You are here:Home » Antibodies » Abs to Chromatin Proteins » Anti -BAF57 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily E Member 1, BRG1-associated Factor 57, BAF57, SMARCE1)

Anti -BAF57 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin
Subfamily E Member 1, BRG1-associated Factor 57, BAF57, SMARCE1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Catalog #B0003-63P3
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.2-0.6ug/ml
Immunohistochemistry (paraffin): 0.2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHEPG2, HeLa and Jurkat
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-PPTDPIPEDEKKE, from the C-terminus of the protein sequence according to NP_003070.3.
SpecificityRecognizes human BAF57/SMARCE1. Species sequence homology: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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